In vitro immunomodulatory and cytoprotective effects of Origanum vulgare methanol extract

Abstract

Event Abstract Back to Event In vitro immunomodulatory and cytoprotective effects of Origanum vulgare methanol extract Milica Vujicic1*, Ivana Stojanovic1, Ivana Nikolic1, Vassiliki Kontogianni2, Pantelis Charisiadis2, Andreas G. Tzakos2 and Stanislava Stosic-Grujicic1 1 Institute for Biological Research "Sinisa Stankovic", Department of Immunology, Serbia 2 University of Ioannina, Department of Chemistry, Greece In addition to being a spice, Origanum vulgare is recognized as a source of natural antioxidants and exerts anti-fungal, anti-bacterial and anti-inflammatory actions. In this study we focused on the immunomodulatory effects of methanol fraction of Origanum vulgare (oregano extract – OE) and on its influence on cellular viability. The methanol OE was prepared from ground oregano leaves by sequential extraction of four solvents of gradually increasing polarity (hexane, ethyl acetate, dichloromethane and methanol). Our results indicate that 48-hour treatment of spleen mononuclear cells with OE (25 - 100 g/ml) did not alter their basic or concanavalin A-triggered proliferation (MTT assay), as well as IFN-γ and IL-4 secretion determined by ELISA. However, OE treatment decreased IL-17 secretion implying a potential inhibitory effect on Th17 cells. Additionally, OE seemed to affect macrophage cytocidal function since it down-regulated production of nitric oxide (NO) in peritoneal cells (PC) (measured by Griess reaction) without an effect on PC viability. However, PC-related IL-1 and TNF- remained unchanged after OE treatment. Finally, apoptosis measurement by histone-DNA ELISA showed that OE rescued isolated pancreatic islets from cytotoxic effect of cytokines. Furthermore, OE fully preserved beta cell function judging by impaired insulin secretion. The mechanism of OE-exerted protection of pancreatic islets was NO-independent since OE did not change islet NO production. In conclusion, OE alters secretory pattern of immune cells and protects pancreatic islets from cytokine-mediated destruction. The observed immunomodulatory and islet cytoprotective effects of OE are being further explored for potential application for diabetes treatment. Acknowledgements This work was supported by the Project of Ministry of Education and Science, Republic of Serbia (No.: 173013) Keywords: Origanum, antioxidant, Diabetes Mellitus, immunomodulatory, cytoprotective, beta cells, Th17 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Vujicic M, Stojanovic I, Nikolic I, Kontogianni V, Charisiadis P, Tzakos AG and Stosic-Grujicic S (2013). In vitro immunomodulatory and cytoprotective effects of Origanum vulgare methanol extract. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00386 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Milica Vujicic, Institute for Biological Research "Sinisa Stankovic", Department of Immunology, Belgrade, Serbia, micijana@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Milica Vujicic Ivana Stojanovic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas G Tzakos Stanislava Stosic-Grujicic Google Milica Vujicic Ivana Stojanovic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas G Tzakos Stanislava Stosic-Grujicic Google Scholar Milica Vujicic Ivana Stojanovic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas G Tzakos Stanislava Stosic-Grujicic PubMed Milica Vujicic Ivana Stojanovic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas G Tzakos Stanislava Stosic-Grujicic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.