Abstract
BackgroundDental pulp (DP) represents an accessible and valuable source promising of stem cells for clinical application. However, there are some disadvantages associated with the isolation of dental pulp stem cells (DPSCs), which include the size and weight of the pulp tissue needed to yield sufficient cells for culturing in vitro. Therefore, the objective of this study was to compare in vitro histomorphometry of DP from permanent (premolars, third molar), supernumerary and deciduous teeth of patients between 5 and 25 years old with regards to weight, length, width and the cell density in the four regions of the DP in order to obtain quantitative parameters in a tissue that represents a valuable source of stem cells.MethodsDPs were obtained from 10 central incisors deciduous, 20 permanent teeth (10 premolars, 10 third molars) and 10 supernumeraries (six mesiodents and four inferior premolar shapes). The pulps were carefully removed, and the entire tissue was weighed. The pulp length and the width were measured with a digital Vernier caliper. The cellular density analysis was performed according to the four regions of the DP (coronal, cervical, medial and apical) in histological slides using photography and the ImageJ® program for quantification.ResultsThe Pearson correlation test revealed that DP weight among different types of teeth is correlated with age in male patients. A significant positive correlation was noted between length and width of the DP with age in both genders. The mean DP weight for supernumerary and third molar teeth was greater than deciduous and premolar teeth. Finally, the histological analysis showed that the coronal and apical portions of DP in supernumerary and premolar teeth have the highest cell density.ConclusionsThe DP of supernumerary teeth has quantitatively the best morphometric parameters and cell density comparable with the quality of DP obtained from deciduous teeth.
Highlights
Dental pulp (DP) is an innervated, highly vascularized soft tissue that provides vitality to the tooth (Rodas-Junco et al, 2017)
The regenerative function of DP suggests that it contains odontogenic progenitor cells or stem cells that are involved in the regeneration process
There are some disadvantages associated with the isolation of dental pulp stem cells (DPSCs), which might be directly related to the size and weight of DP tissue by limiting the number of stem cells isolated from it (Raoof et al, 2014)
Summary
Dental pulp (DP) is an innervated, highly vascularized soft tissue that provides vitality to the tooth (Rodas-Junco et al, 2017). Until now there are no reports that relate morphometric parameters such as weight, length, width and cellular density of DP among different types of teeth and its influence on tissue quality for the isolation of DP cells. The objective of this study was to compare the histomorphometry of DP in temporal and permanent teeth and evaluate the cell density in four regions in this tissue with to purpose of generating quantitative parameters that would have important applications in the DPSCs isolation. The objective of this study was to compare in vitro histomorphometry of DP from permanent (premolars, third molar), supernumerary and deciduous teeth of patients between 5 and 25 years old with regards to weight, length, width and the cell density in the four regions of the DP in order to obtain quantitative parameters in a tissue that represents a valuable source of stem cells. Conclusions: The DP of supernumerary teeth has quantitatively the best morphometric parameters and cell density comparable with the quality of DP obtained from deciduous teeth
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