In Vitro Growth Responses of Ornamental Bananas (Musa sp.) as Affected by Light Sources

Wagner A Vendrame,Paulo Mauricio Centenaro Bueno,Cassandre Feuille,David Beleski

doi:10.3390/horticulturae8020092

Wagner A Vendrame, Paulo Mauricio Centenaro Bueno + Show 2 more

Open Access

https://doi.org/10.3390/horticulturae8020092

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Abstract

Light-emitting diodes (LEDs) have become very popular for the production of horticultural crops. LEDs represent an alternative lighting source to regular fluorescent (FL) bulbs, increasing the quality of plants and minimizing production costs. LEDs also provide selective light intensity and quality, suitable for commercial micropropagation. The objective of this study was to evaluate the growth and development of in vitro ornamental bananas under different light sources. Two ornamental banana varieties were selected for this study: Musa ‘Little Prince’ and Musa ‘Truly Tiny’. Light quality and intensity of three different light sources were evaluated: LED-1 (116 μmol m−2 s−1), LED-2 (90 μmol m−2 s−1), and FL (100 μmol m−2 s−1). Length and biomass of plantlets were greater under LED-1 compared to FL but not significantly different from LED-2. The fresh and dry weight of shoots and roots, number of leaves, and number and length of roots were not significantly different between treatments. Chlorophyll content was greater under LEDs. Leaf number and stomata number and size were greater under FL. Our results indicate that shoot length and biomass could be improved by optimizing light quality and intensity. Different responses to light sources between the two banana varieties also indicated a genotype effect.

Highlights

Baby food jars with polypropylene lids were sealed with parafilm, and cultures were placed in a growth chamber under controlled environmental conditions of 27 ± 2 ◦ C and a 16 h photoperiod at 100 μmol m−2 s−1 PPFD
The light source had no significant effect on most in vitro growth and development parameters evaluated for both banana varieties, including stem diameter, shoot and root fresh weight, leaf number, shoot and root length, and shoot and root dry weight (Table 1)
Our results revealed that shoot mass and length could be promoted by controlling light quality and intensity

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Light is one of the primary factors that affect in vitro plant morphogenesis [1,2]

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