In vitro growth response of Patchouli (Pogostemon cablin) cultured in medium containing Methylobacterium spp. filtrate

Abstract

Mass seeds production through micropropagation can support modern agriculture, but this technique is expensive and largely determined by a medium formulation. The addition of synthetic Plant Growth Regulator (PGR) has a residual effect, meanwhile natural PGRs is more secure but expensive. In vitro testing of PGR activity obtained from bacteria was the first step to substituted synthetic PGRs to become nature PGR. Research to observe PGR activity obtained from four strains of Methylobacterium spp that had been analyzed of the capability of PGRs synthesis was done on in vitro patchouli cultured. There are three activities: 1) The sterilized technique of filtrate using filtration techniques, humid heat, and a combination of both; 2) Testing of Methylobacterium spp. from that production of cytokinins and compared its activity with commercial PGRs (BA and zeatin); and 3) auxin (NAA and IAA). The research showed that strain TD-J2 filtrate added to medium was similar to BA activities, and TD-J7 was similar to zeatin. Auxin activity derived from strain TD-J10 showed the capacity to induces root formation. The research result indicates that synthesis PGR derived from Methylobacterium spp. has similar activity with commercial PGRs.