Abstract
Summary Flurprimidol (FLP) is an experimental gibberellin biosynthesis inhibitor whose biological properties have not been fully characterized. Addition of FLP (0.5-2.0 mg 1-1) to a callus medium reduced both fresh and dry weights of moth bean (Vigna aconitifolia) callus after 21 days. The concentrations (fresh weight basis) of total sugar, proline, soluble protein, and phenols in callus tissue were all increased by FLP relative to the control. In addition, the activities of peroxidase, protease, RNase, alanine aminotransferase, and aspartate aminotransferase were all greater in FLP-treated cultures than in controls. The presence of 1 mg 1-1 FLP in a regeneration medium reduced the percentage of cultures exhibiting roots as well as the mean number of roots formed per culture. Likewise, FLP reduced the formation of green meristematic nodules which are forerunners of shoots in moth bean callus.
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