IN VITRO GLYCOLYSIS OF WHOLE BLOOD CAN DETECT PRIMED NEUTROPHILS IN SEPTIC ICU PATIENTS

Abstract

The purpose of this study was to develop a bedside assay based on the in vitro glycolysis of a whole blood sample that could detect primed neutrophils (PMNs). A mathematical index of the PMN response to exogenous stimulation with phorbol myristate 13-acetate (PMA), called the Delta value, was derived by comparing the increase in glycolysis for paired blood samples with and without PMA to that expected from normal subjects. Delta values for systemic inflammatory response syndrome/sepsis patients (9.09 ± 7.61) (N = 36) were significantly higher than normal controls (2.02 ± 1.76) (N = 51), nonsepsis ICU patients (3.81 ± 2.80) (N = 14) and patients in septic shock (2.33 ± 3.04) (N = 10) (p < .05). Delta values were consistently reflected in parallel measurements of increased reactive oxygen species production by neutrophils detected cytofluorometrically. PMN priming can be simply and rapidly detected by an assay based on the numbers of PMNs and erythrocytes and the measured rates of in vitro glycolysis of paired whole blood samples with and without PMA.