In vitro germination of seeds and acclimatization of Macambira (Bromelia laciniosa Martius ex Schultes f.) seedlings

Abstract

The macambira is an ornamental plant that has a high degree of ecological importance in ecosystems. This study aimed to evaluate the in vitro germination of macambira due to different culture media and establish the best substrate for the acclimatization of micropropagated plants. For that were tested five treatments, 100, 75, 50, 25 and 0 of the MS medium. We estimated the percentage of germination and germination speed index (GSI). For acclimation were evaluated five combinations of substrates: coconut coir fiber: earthworm humus (2:1); coconut coir fiber: earthworm castings: sand (1:1:1) , (2:1:1) and (2:2:1), and vermiculite : earthworm humus (2:1). Was made to ANOVA and means were compared by Tukey test at 5%. There was no significant difference between the concentrations of MS salts as the percentage of germination. As for abortion, the treatment 100 % MS salts showed the worst results. The acclimatization of seedlings for the variables, number of leaves and dry weight of roots and shoots no significant difference. For the variable length of the longest root, coconut coir fiber: earthworm humus (2:1) and coconut coir fiber: earthworm castings: sand (2:1:1) and (2:2:1) showed the highest average and dry root the coconut coir fiber: earthworm castings: sand (1:1:1) and (2:2:1) provided the best results. The germination can be carried out in vitro in a medium containing only water and agar to acclimatization and must use the substrate coconut coir fiber: earthworm compost: sand (2:2:1).