Abstract
The production of antierythrocyte autoantibodies was studied by a plaque assay. Plaque-forming cells (PFC) and hemagglutinating antibodies were produced upon in vitro culture of murine spleen cells in the presence of mouse red blood cells (MRBC). Kinetic experiments revealed that the plaque response reached a maximum after 5 to 6 days of culture. The generation of PFC required the presence of MRBC at the beginning of the culture. PFC numbers were highly increased when 1% horse serum was added to the culture medium. Horse serum was found to sensitize the plaque assay by interacting directly with MRBC. After filtration of horse serum on Sephadex G-200 columns, only the fraction eluting in the void volume increased PFC numbers and interacted with MRBC.
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