In vitro gas and methane production of two mixed rations influenced by three different cultures of Saccharomyces cerevisiae

M M Y Elghandour,J C Vázquez,A Z M Salem,A E Kholif,M M Cipriano,L M Camacho,O Márquez

doi:10.1080/09712119.2016.1204304

Abstract

ABSTRACTThe current study aimed to evaluate if the effect of Saccharomyces cerevisiae (SC) on in vitro fermentation can be affected with the crude protein (CP) content of the ration. Three commercial SC cultures of Biocell F53®, Procreatin 7®, and Biosaf SC47® were evaluated at 0 (SC0), 2 (SC2), and 4 (SC4) mg/g dry matter (DM) of substrate. Two rations with 13% (low crude protein [LCP]) and 16% CP (high crude protein [HCP]) were used as substrates. Rumen gas (gas production [GP]) and methane (CH4) productions were recorded. The HCP ration had increased (P = .05) asymptotic GP, CH4 production, and fermentation parameters. Biocell F53® and Biosaf SC47® increased the asymptotic GP (P < .05) in HCP and LCP rations with better effect for the dose of 2 mg/g DM substrate HCP (P < .05) and dose of 4 mg yeast/g DM substrate with the LCP ration. The highest CH4 production was observed (P < .05) with Procreatin 7®. It could be concluded that HCP ration improved GP than LCP ration. Moreover, addition of Biocell F53® and Biosaf SC47® at rate of 2 mg/g DM improved fermentation kinetics and nutrients degradability.Abbreviations:b: the asymptotic gas production; c: the rate of gas production; CH4: methane; GY24: gas yield at 24 h of incubation; L: the initial delay before gas production begins; MCP: microbial CP production; PF24: partitioning factor at 24 h of incubation.

Highlights

One of the most important problems facing ruminant production is the losing of energy and high biological value proteins as a result of ruminal fermentation
Researches are concerning the use of natural feed additives, generally recognized as safe for human consumption, including phytogenic extracts (Valdes et al 2015), enzymes (Alsersy et al 2015) or Saccharomyces cerevisiae (SC) (Elghandour, Salem et al 2015) to modify rumen microbial fermentation
Two mixed rations with two different levels of crude protein (CP) of 13% (LCP) and 16% (HCP) on dry matter (DM) basis (Table 1) were used as substrates to be incubated with three doses of different SC cultures

Summary

Introduction

One of the most important problems facing ruminant production is the losing of energy and high biological value proteins as a result of ruminal fermentation. The SC have the ability to increase dry matter (DM) and neutral detergent fibre (NDF) digestion (Elghandour et al 2014, Elghandour, Salem et al 2015), and increase initial rates of fibre digestion (Williams et al 1991) It could enhance fungal colonization of plant cell walls, resulting in increased DM and NDF digestion (NDFD) (Patra 2012), and improved in situ crude protein (CP) and NDF degradation. It could enhance fungal colonization of plant cell walls, resulting in increased DM and NDF digestion (NDFD) (Patra 2012), and improved in situ crude protein (CP) and NDF degradation. Elghandour et al (2014) reported an increased in vitro rumen degradability of forages, which was associated with ability of yeast to stimulate growth and activity of fibrolytic bacteria (Wambui et al 2010)

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Conclusion