In vitro Free Radical Scavenging Potential of Defatted Ethanolic Extract of the Seeds of Lepidium sativum Linn.

Abstract

Aim The objective of the present study was to evaluate the antioxidant potential of the defatted ethanolic extract of the seeds of Lepidium Sativum Linn. Methods Different in vitro chemical assays viz. DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging, ABTS (2, 2- azinobis- (3-ethylbenzothiazoline-6-sulphonate) radical scavenging, iron chelation, lipid peroxidation, superoxide scavenging and non-enzymatic haemoglobin glycosylation assay were used. The total antioxidant capacity of the extract was determined spectrophotometrically by phosphomolybdic acid method. Results & Conclusion The defatted lepidium seed extract showed significant free radical scavenging activity in ABTS and non-enzymatic glycosylation assays, and a moderate activity in all the other assays. IC 50 of the extract in the DPPH, ABTS, iron chelation, lipid peroxidation and super oxide scavenging assays were found to be 171.13, 38.64, 128.94, 71.39 and 206.09 μg·mL −1 respectively. The haemoglobin glycosylation assay of the extract showed a percentage scavenging of 46.60% and 74.88%, at 0.5 and 1.0 μg·mL −1, concentrations, respectively. Total antioxidant capacity of ethanolic extract of L. sativum (10 mg·mL −1) was found to be equivalent to 58.38 μg·mL −1 of ascorbic acid.