Abstract
Antioxidant activity of the methanol extract of Ixora coccinea L. was determined by DPPH free radical scavenging assay, reducing power and total antioxidant capacity using phosphomolybdenum method. Preliminary phytochemical screening revealed that the extract of the flower of I. coccinea possesses flavonoids, steroids and tannin materials. The extract showed significant activities in all antioxidant assays compared to the standard antioxidant in a dose-dependent manner and remarkable activities to scavenge reactive oxygen species (ROS) may be attributed to the high amount of hydrophilic phenolics. In DPPH radical scavenging assay the IC50 value of the extract was found to be 100.5 μg/mL while ascorbic acid had the IC50 value 58.9 μg/mL. Moreover, I. coccinea extract showed strong reducing power and total antioxidant capacity.
Highlights
In living systems, free radicals are generated as part of the body’s normal metabolic process, and the free radical chain reactions are usually produced in the mitochondrial respiratory chain, liver mixed function oxidases, through xanthine oxidase activity, atmospheric pollutants and from transitional metal catalysts, drugs and xenobiotics
As a part of our ongoing investigations about natural anti-oxidants from local medicinal plants of Bangladesh (Alam et al, 2008a; Alam et al, 2008b), in this paper, we have reported the antioxidant activity of the flowers of I. coccinea
Prepared DPPH solution (0.004% w/v) was taken in test tubes and I. coccinea extracts was added followed by serial dilutions (1 to 500 μg) to every test tube so that the final volume was 3 mL and after 10 min, the absorbance was read at 515 nm using a spectrophotometer (HACH 4000 DU UV–visible spectrophotometer)
Summary
Free radicals are generated as part of the body’s normal metabolic process, and the free radical chain reactions are usually produced in the mitochondrial respiratory chain, liver mixed function oxidases, through xanthine oxidase activity, atmospheric pollutants and from transitional metal catalysts, drugs and xenobiotics. Oxygen free radical can initiate peroxidation of lipids, which in turn stimulates glycation of protein, inactivation of enzymes and alteration in the structure and function of collagen basement and other membranes, and play a role in the long-term complication of diabetes (Sabu and Kuttan, 2002; Boynes, 1991; Collier et al, 1990). Synthetic antioxidants such as butylated hydroxytoluene, butylated hydroxyanisole, and tertbutylhydroquinone have been commonly used as antioxidants in foods for years, their safety has long been questioned (Branen, 1975; Ito et at., 1983). The evaluation of antioxidant power was performed in vitro by the DPPH (1, 1-diphenyl, 2picrylhydrazyl), reducing power and total antioxidant capacity assays
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