In Vitro Flowering from Cultured Nodal Explants of Rose (Rosa hybrida L.)

Abstract

Roses are one of the worlds most important ornamentals for a long time and are most often used for ornamental, medicinal and aromatic purposes. The study reports in vitro multiple shoot formation and flower induction of Rosa hybrida L. cv. Red Masterpiece with maximum number of 5 shoots per explant on MS medium supplemented with 3 mg/l BA and 1 mg/l kinetin, followed by flower induction on MS medium containing 2 mg/l BA for 9 weeks. The shoots readily rooted on MS medium devoid of growth regulators. Shoots cultured under various photoperiods did not flower. Rooted plantlets were hardened and established in pots with 100% survival.