Abstract
Thin cellular layers of epidermal, subepidermal and cortical tissues were taken from floral pedicels and peduncles of the unripe infructescences of Nicotiana tabacum cv. White Burley doubled haploid plants. These plants belong to an isogenic line with abundant in vivo flowering, and their pedicel explants show in vitro, compared with those of amphidiploid control, a higher capacity to differentiate flowers in a short period of time and in the near or complete absence of vegetative competition. These results may provide elements for using suitably selected doubled haploid plants in order to study floral differentiation and its genetic control.
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