In vitro fibroblasts viability and migration stimulation of Acalypha indica: an insight on wound healing activity

Ammar Mahmoud Ibrahim,Amir Husni Mohd Shariff,Rama Ali Althiab,Mariani Abdul Hamid,Razauden Mohamed Zulkifli

doi:10.1186/s43094-021-00333-0

Ammar Mahmoud Ibrahim, Amir Husni Mohd Shariff + Show 3 more

Open Access

https://doi.org/10.1186/s43094-021-00333-0

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Abstract

BackgroundThe current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate.ResultsAerial parts extract exhibited higher DPPH scavenging activity compared to root extract with IC50 of 62 µg/mL and 206 µg/mL, respectively. Both aerial parts and root extracts showed low cytotoxicity towards fibroblasts with 753 µg/mL LD50 for aerial parts and undetected LD50 for root extract. Additionally, aerial parts extract significantly induces fibroblasts proliferation up to 134%. Wound closure investigation showed a significant closure percentage for aerial parts compared to untreated control with 75% at 1 µg/mL and high closure percentage with 70% at 0.1 µg/mL for root extract compared to only 59% closure percentage for untreated control after 48 h of the study.ConclusionsThis study provided evidence for A. indica to have great wound healing potential. The finding builds the scientific background in future to utilise the high antioxidant activity of A. indica and its ability to stimulate fibroblasts migration and proliferation for further applications.

Highlights

This study aims to validate the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate
Referring to IC50 values, Table 1 confirms higher antioxidant activity against DPPH for aerial parts extract compared to root extract
We found that ethanolic extracts of both aerial parts and root have high antioxidant activity, support fibroblasts viability, and accelerate fibroblasts migration

Summary

Introduction

The current study investigates the antioxidant activity of Acalypha indica aerial parts and root ethanolic extracts and explore whether these extracts will stimulate fibroblasts viability and ability to migrate. Ancient civilisations exploited plant-based treatments as the primary source for remedies to cure diseases [1]. Through decades, those traditional remedies have proved efficacy even without any scientific justification. Traditional healers used A. indica for wound healing management [5]. Studies on A. indica identified abundant varieties of second metabolites such as glycosides, triterpenes, amides, tannins, cyanogenic glucoside acalyphin, including antioxidant entities such as Acalyphamide (flavonoid) and Acalyphine (alkaloid) [6,7,8,9].

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