Abstract
<p><strong>Introduction</strong></p><p>Testicular epididymal sperm aspiration (TESA) is one of the method to retrieve sperm from the testes in men with azoospermia. The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa.</p><p><strong>Material &amp; Methods</strong></p><p>A retrospective comparative analysis of patients who underwent fresh TESA and frozen-thawed TESA in ICSI-ET cycles from January 2012 to December 2014 in Halim Fertility Center was done. Fresh testicular epididymal sperm aspiration (fresh TESA) was performed on the same day with oocyte retrieval in 28 cycles and the frozen-thawed testicular epididymal sperm aspiration (frozen-thawed TESA) was used in 30 cycles. </p><p><strong>Results</strong></p><p>The two groups were comparable in terms of the ages of male and female patients, etiology of infertility and duration of infertility. Fertilization rates in fresh TESA group were 53,5% and in frozen-thawed TESA group, fertilization rates were 50%. There was no statistically significant difference between the groups. Clinical pregnancy rates in fresh TESA group were 35,7% and in frozen-thawed TESA group, clinical pregnancy rates were 26,7% and statistically there was no significant difference between the groups.</p><p><strong>Conclusion</strong></p>There is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA .
Highlights
Testicular epididymal sperm aspiration (TESA) is one of the method to retrieve sperm from the testes in men with azoospermia
It soon became clear that intracytoplasmic sperm injection (ICSI) could be used for couples in which the male partner has a limited number of spermatozoa in the ejaculate, or for epididymal and testicular spermatozoa [1]
We found that there was no statistically significant difference in no.of oocytes collected, fertilization rates and clinical pregnancy rates between fresh TESA and frozen-thawed TESA
Summary
Testicular epididymal sperm aspiration (TESA) is one of the method to retrieve sperm from the testes in men with azoospermia. There is no significant difference in the in vitro fertilization outcome of intracytoplasmic sperm injection (ICSI)-ET cycles between fresh TESA and frozen-thawed TESA. Testicular epididymal sperm aspiration (TESA) can be used to retrieve sperm from the testes either in men with OA failed to do PESA as well as in men with non-obstructive azoospermia (NOA). The aim of the study is to compare the In vitro fertilization (IVF) outcome of intracytoplasmic sperm injection (ICSI)-ET cycles with fresh testicular epididymal spermatozoa obtained on the same day with oocyte retrieval and with frozen-thawed testicular epididymal spermatozoa
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