IN Vitro Experimental Evaluation of Antiviral and Anticancer Potentials of Both Orange Albedo and Grape Seed Extracts

Abstract

The present work aimed to evaluate the antiviral and anticancer potentials of reusable orange albedo extract (OAlbE) and grape seed extract (GSE) wastes. Results revealed that the IC50 values of OAlb M/E and GSE M/E extracts with MCF-7 cell lines were 184439, 48108, 344,11.68 µgm / ml and was 6016 µgm / ml for SGSE 24 hrs post treatment respectively while the IC50 values of the extracts with Caco-2 cell lines were 23708, 14107, 1158,15607 µgm / ml and was 430 µgm / ml for SGSE 24 hrs post treatment, respectively . The SGSE was of a higher potential toxicity to experimental extracted one. Antiviral activity of E/M OAlbE and GSE showed the same antiviral potential against RVFV recording 0.51 log (10) / 0.1 ml ; (6.58%) and 1 log (10) / 0.1 ml (12.9%) respectively. While the antiviral potential against HAV revealed that OAlbE/M and GSME showed low potential against HAV recording 0.26 log (10) /0.1 ml. On the contrary both O-AlbE and GSME showed no antiviral potentials compared with Standard IFN (5 IU/ml) recorded a depletion of viral infectivity titers recording 3.25 log (10) / 0.1 ml (41.93%) and 3 log (10) /0.1 ml (40%) for RVFV and HAV respectively. Also, anticancer potentials was proved as there was a remarkable BCL-2 , P53 and Bax genes expression compared with control respectively. It can be concluded that both E/M OAlb and GSE are promising antiviral and anticancer agents. More intensified investigations must be conducted to maximize the biological potentials of both extracts. A higher level of characterization of extract contents must be achieved and evaluated for targeting the most promising fraction has both bioactivities considered