Abstract
An interesting property for many probiotic strains is to exhibit antimicrobial activity against pathogenic microorganisms. Therefore, the antimicrobial activity of the potentially probiotic strain Lactobacillus acidophilus L12 against Escherichia coli, Staphylococcus aureus and Salmonella sp. was examined in vitro through co-culturing of Lactobacillus acidophilus L12 with each of the pathogenic strains for 72 h. When cultured individually, all strains reached a final population always exceeding 1012 cfu/mL. When co-cultured with each of the pathogenic strains Lactobacillus acidophilus L12 population always exceeded 1012 cfu/mL, while the bacterial population of the pathogenic strains was reduced in a strain-specific manner. Lactobacillus acidophilus L12 demonstrated the highest inhibitory activity against the two Staphylococcus aureus strains, followed by the two E. coli strains, and the two Salmonella sp. strains tested. The kinetics of the co-culturing process of Lactobacillus acidophilus L12 with each of the test pathogenic strains was modelled using the logistic curve model. The obtained results show the strong antagonistic activity of the potentially probiotic strain Lactobacillus acidophilus L12, thus making it a promising candidate for inclusion in the composition of probiotic preparations and functional probiotic foods.
Highlights
An interesting property for probiotic strains is to exhibit antimicrobial activity against pathogenic microorganisms so that they can play their protagonistic role in the human gastrointestinal tract and reduce the number of harmful bacteria and restore the balance of the gastrointestinal microflora [2, 3]
The bacteriocin gasericin A was isolated from Lactobacillus gasseri
Several Lactobacillus acidophilus strains are Generally Recognized as Safe by the FDA and benefit from a Qualified Presumption of Safety status in EU., Lb. acidophilus bacteria are included in a wide range of probiotic preparations and probiotic foods marketed
Summary
Lactobacillus acidophilus strains have already been reported to adhere to the surface of intestinal epithelial cells. This adhesion is strictly specific to each individual [4]. The bacteriocin gasericin A was isolated from Lactobacillus gasseri. It shows high inhibitory activity against a wide range of food pathogenic bacteria such as Listeria monocytogenes, Bacillus cereus and Staphylococcus aureus. Gasericin A is a highly hydrophobic bacteriocin. This is the first cyclic bacteriocin isolated from the group of lactic acid bacteria [5, 6]
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