In vitro evaluation to intensify the differential morphogenetic response through plant growth regulators and antibiotic supplementation in sugarcane

Abstract

Sugarcane has always witnessed a lower multiplication rate following conventional method of adoption for newly released variety on large scale by farmers. Tissue culture derived techniques proved as effective, reliable and alternative strategy for rapid production of quality planting materials. In vitro morphogenetic responses varies at genotype basis. Thus, an in vitro investigation was conducted to intensify the differential morphogenetic response through plant growth regulators and antibiotic supplementation in two sugarcane genotypes viz. Co 05011 and Co 0118. The cultures were initiated by explant inoculation on basal MS media and further transferred on different media formulations modified with BAP, Kn, TDZ and GA3. The best morphogenetic response was observed in media M21 [1.5 mg/L (BAP and Kn), 0.25 mg/L (TDZ and GA3)] in genotype Co 05011 whereas, genotype Co 0118 showed the best in media M22 [1.5 mg/L (BAP and Kn), 0.25 mg/L TDZ and 0.5 mg/L GA3]. The higher culture survival percent with sterile shoots was observed in 0.05% Cefotaxime fortified media. Findings of study suggests TDZ as an effective PGR for rapid and high frequency shoot regeneration and the supplementation of 0.05% Cefotaxime in media might enhance sterile culture percent.