Abstract
Cariostatic treatment has been shown to successfully arrest caries. However, it blackens the carious tooth structure. This study evaluated the effects of an experimental cariostatic agent with silver nanoparticles (Ag-Nano) using microhardness (MH) and microbiological tests. The cariostatic agents tested were: Saforide®, Cariestop®, Ancarie® and Ag-Nano. Sixty-six samples from deciduous enamel were submitted to initial (after pH cycling to obtain initial caries-like lesion) and final (after cariostatic application) MH testing and %MH values were calculated. After longitudinal sectioning, internal (I) MH was evaluated. Strains of Streptococcus mutans, Escherichia coli, and Enterococcus faecalis in brain-heart infusion culture were treated with the cariostatic agents. Agar diffusion tests (ADTs) were performed and minimum inhibitory concentrations were determined. The statistical tests used were: Kruskal-Wallis and Dunn (%MD; ADT; MIC) and ANOVA followed by Tukey's test (I-MH) (p<0.05). The %MH of Saforide® was significantly greater than that of Ag-Nano (p<0.05). Internal MH showed progressive improvement in the enamel remineralization for all cariostatic tested. In ADTs showed greater inhibition of S. mutans, E. faecalis, and E. coli by Saforide® than by Ancarie® and Ag-Nano. Ag-Nano was able to inhibit 100% microorganism growth at a lower concentration than required for the other agents. It was concluded that Ag-Nano treatment promoted remineralization of deciduous tooth enamel with initial caries-like lesion and bactericidal activity.
Highlights
The existing standard of care for early childhood caries (ECC) consists of surgical and restorative treatments, with little emphasis on prevention and management of the disease [1]
The Agar diffusion tests (ADTs) results are presented in Table 3; all cariostatic activity [7]
In relation to Minimum Inhibitory Concentration (MIC), all cariostatic tested in this study cariostatic agent with silver nanoparticles, without staining were able to inhibit the microorganisms
Summary
The existing standard of care for early childhood caries (ECC) consists of surgical and restorative treatments, with little emphasis on prevention and management of the disease [1]. The study hypothesis was that the tested cariostatic agent with silver nanoparticles would show remineralization potential in deciduous dental enamel and bactericidal activity against S. mutans, Escherichia coli and Enterococcus faecalis. Analysis of Internal Microhardness To evaluate the internal effects of the cariostatic agents on dental enamel, blocks in G1-G6 were sectioned along their long axes with a diamond disc in a precision cutter (Isomet 1000; Buehler Ltd., Lake Bluff, IL, USA).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
