In Vitro Evaluation of The Inhibitory Power of Trichoderma harzianum Against Pathogens that Cause Anthracnose in Chili

Abstract

This study aims to determine the inhibition of Trichoderma harzianum Tc-Jjr-02 isolates against two Colletotrichum isolates that cause anthracnose in chili in vitro. The first stage of the test was carried out by a double culture method on the agar dextrose-chloramphenicol medium with five replications. Inhibition of biocontrol agents was determined by comparing the difference between growth of pathogenic colonies in multiple cultures and monoculture with growth of monoculture pathogenic colonies measured every 24 hours for six days. At two weeks after the end of the Observation Test the second stage of research was carried out. Mycelium biocontrol agents that overlap with pathogenic mycelium isolates are sampled and observed under a microscope with 400 times magnification. In vitro test results showed that T. harzianum Tc-Jjr-02 isolates were able to inhibit the growth of C. capsici and C. gloeosporioides colonies with an average of 64.2 ± 3.54 and 65.0 ± 3.93%, respectively. On microscopic observations of overlapping mycelium it appears that T. harzianum Tc-Jjr-02 damages the C. capsici and C. gloeosporioides hyphae cell walls. T. harzianum has the potential to be an effective biocontrol agent against pathogens that cause chili anthracnose.