IN VITRO EVALUATION OF THE CYTOTOXIC POTENTIAL OF Securidacalongepedunculata ON HUMAN BREAST ADENOCARCINOMA (MCF‐7) CELLS

Abstract

The prevalence of cancer has increased interest in the role of complementary and alternative medicine, employing the use of plant products. Securidacalongepedunculata (SL) serves as a major component of anticancer decoctions in Nigeria. The present study evaluates the cytotoxic potential of the crude extract and fractions of SL root bark against human breast adenocarcinoma (MCF‐7) cell line. The root back of SL was pulverized and extracted with 80% methanol to yield a crude extract which was then submitted to liquid–liquid fractionation with dichloromethane (DCM) and butanol (BUT). The extract and fractions were subjected to Gas Chromatography‐Mass Spectrometry (GC‐MS). MCF‐7 cell line was treated with graded concentrations (9.77 – 5000 μg ml−1) of the aqueous methanol crude extract as well as the DCM and BUT fractions for 24 or 48 hours. Cell viability was thereafter measured by XTT proliferation assay. The activity of the aqueous methanolic crude extract was found to be both dose‐dependent and time independent with IC50 values of 2241μg ml−1 and 1808 μg ml−1 after 24 and 48 hours respectively. However, the DCM and BUT fractions were significantly (p < 0.05) cytotoxic with IC50 values of 86.27 μg ml−1 and 12.08 μg ml−1 respectively after 48 hours of treatment. The study lends credence that BUT fractions of SL may contain lead agent with anticancer properties and that informed its use in alternative medicine.Support or Funding InformationSELF FUNDINGThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.