‘In vitro’ evaluation of the antioxidant activity in relation with structure and kinetic properties of scleraldehyde

Abstract

Schizophyllum commune is the world′s most extensively disseminated mushroom except in Antarctica. Schizophyllan is a polysaccharide fractions isolated from S. commune. The schizophyllan is oxidized to form scleraldehyde to introduce carbonyl group for additional functions. The relationship between structure, kinetic and antioxidant properties of scleraldehyde was studied in order to establish the molecular characteristics related to its maximum radical scavenging activity. In our earlier paper we have reported that scleraldehyde obtained from schizophyllan with the dialdehyde content of 28%. From the absorption studies, it explains there is π–π⁎ excitations involving the benzene groups. Moreover, the structural identification of scleraldehyde were characterized by bands at 1670 and 3600–3200 cm−1, corresponding to the axial deformation of the C=O bond and the angular deformation of the C–H bond respectively. The IR spectrum confirms the identity of aldehyde. The scavenging assays of scleraldehyde was found to be reliant on their reactivity and concentration. Scavenging assays performed indicate that 100 μM of scleraldehyde showed the highest antioxidant activity compared to the ascorbic acid. Furthermore, The reducing ability of the scleraldehyde was 54.81±0.92 μM of Fe(II)/g compared to the control (Ascorbic acid) respectively. The kinetic mechanism of scleraldehyde portrays a competitive inhibition against antioxidant enzymes on calculating for radical scavenging activity. By means of structure determination, kinetic assays and the antioxidant assays that hydroxyl group present in C2, C4 and C6 are the main reactive sites. The hypothesis converse that scleraldehyde can serve as scavenger of aqueous peroxyl radicals near the membrane surface of collagen.