IN VITRO EVALUATION OF SOME BRANDS OF DICLOFENAC SODIUM INJECTION MARKETED IN GREATER NOIDA, UTTAR PRADESH, INDIA USING UV-VIS SPECTROPHOTOMETRIC METHOD

Abstract

Quality is of principal subject to human beings in all perspective of life. When it comes to the quality of the pharmaceuticals which are consumed by humans, it is of most extreme significant as they are utilized for the prosperity of the humanity. In this study our objective was to evaluate the experimental in vitro study of the commercially obtainable Diclofenac Sodium injection brands which are available in Greater Noida. Ten different ampoules of 75mg/3ml diclofenac sodium injection products randomly collected from retail pharmacies and Govt. Hospital of targeted area in Greater Noida, Uttar Pradesh, India, twenty ampoules 75 mg/3ml have the same batch number of each brand were collected for the analysis. All the analytical methods were done in the quality control laboratories of JBJM Parenteral Pvt Ltd, Greater Noida, Uttar Pradesh, India. The study was carried out by performing various tests which is affiliated with the quality such asgeneral appearance of Diclofenac Sodium injection ampoule, Particulate matter, Average fill volume, pH and content assay through pH Meter (Spectra lab), UV-Vis Spectrophotometer (UV-1900i SHIMADZU). The assay was evaluated by UV-Vis Spectrophotometer at the wavelength of 276nm. The results show that diclofenac sodium injection following IP specification, it can be concluded that all of the 10 brands of the drug passed through UV-Vis spectroscopy method also in pH meter. The Spectroscopy method was successfully applied to evaluate of Diclofenac Sodium injection. KEYWORDS: In vitro evaluation, Diclofenac sodium injection, UV-Vis Spectroscopy, pH, Analytical method, Pharmaceutical analysis, SHIMADZU, Greater Noida, Spectroscopy, Quality evaluation