Abstract
Introduction: Selligueain A derived from the roots of Polypodium feei was shown to have anti-inflammatory activity, which was tested in vivo on the rats’ paw edema induced by carrageenan. The aim of this study was to evaluate the anti-inflammatory mechanism of selligueain A in vitro against the production of pro-inflammatory mediators. Methods: In this study, RAW264.7 cells were used as an inflammatory cell model, and observations were made on the inflammatory mediators nitric oxide (NO), inducible nitric oxide synthase (iNOS), and tumour necrosis factor-α (TNF-α). The NO concentration was measured by the Griess reaction, and the iNOS enzyme and the TNF-α concentrations were determined by the ELISA method. Cell viability was assessed by the [3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) test. Results: Selligueain A at concentrations of 100 and 150 µM suppressed the production of NO, iNOS, and TNF-α in RAW264.7 cells stimulated by lipopolysaccharide (LPS). The concentration of 150 µM showed the highest inhibition of NO, iNOS, and TNF-α mediators with the percentage inhibition of 64.85, 55.01, and 48.54%, respectively. Conclusion: This study shows that selligueain A has anti-inflammatory activity through inhibition of NO, iNOS, and TNF-α production in RAW264.7 macrophage cells.
Highlights
Selligueain A derived from the roots of Polypodium feei was shown to have antiinflammatory activity, which was tested in vivo on the rats’ paw edema induced by carrageenan
They are the main source in producing pro-inflammatory cytokines such as tumour necrosis factor-α (TNF-α), nitric oxide (NO), interleukins (IL) and inflammatory mediating enzymes such as cyclooxygenase (COX) and inducible nitric oxide synthase [2]
Identification of selligueain A Selligueain A is a known compound of the P. feei roots
Summary
Selligueain A derived from the roots of Polypodium feei was shown to have antiinflammatory activity, which was tested in vivo on the rats’ paw edema induced by carrageenan. Methods: In this study, RAW264.7 cells were used as an inflammatory cell model, and observations were made on the inflammatory mediators nitric oxide (NO), inducible nitric oxide synthase (iNOS), and tumour necrosis factor-α (TNF-α). Conclusion: This study shows that selligueain A has anti-inflammatory activity through inhibition of NO, iNOS, and TNF-α production in RAW264.7 macrophage cells. The response of inflammation has an important role in the pathogenesis of several diseases initiated by tissue injury and caused by the invasion of pathogenic microorganisms or free radicals that can cause vascular and cellular reactions. Macrophages play an important role in the inflammatory response They are the main source in producing pro-inflammatory cytokines such as tumour necrosis factor-α (TNF-α), nitric oxide (NO), interleukins (IL) and inflammatory mediating enzymes such as cyclooxygenase (COX) and inducible nitric oxide synthase (iNOS) [2].
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