In vitro evaluation of nematicidal properties of Solanum sisymbriifolium and S. nigrum extracts on Pratylenchus goodeyi

Abstract

The root-lesion nematode, Pratylenchus goodeyi, is among the most economically damaging parasites of banana plants. Nematode control can benefit from searching for novel bio-nematicides. The present study was carried out to assess the potential nematicidal properties of two Solanum species (Solanum sisymbriifolium and S. nigrum) against P. goodeyi, using dichloromethane, acetone, ethanol and either cold or hot water extracts of the plants. Water extracts of both plants at a concentration of 10 mg ml−1 greatly affected nematode movement and also caused mortality. The analysis of sequential extracts at the same experimental concentrations showed that, although water extracts affect nematode mobility and mortality, the acetone extract from S. nigrum was the most efficient, causing 100% mortality after 23 h exposure. The results showed that S. sisymbriifolium and S. nigrum extracts contain chemical components that induce morphological changes in the body structure of the root-lesion nematode, affect mobility and cause mortality. The nematostatic and nematicidal potential of the extracts described herein merit further studies to find novel bio-nematicides against the root-lesion nematode.