Abstract
Objective: The purpose of this study was to evaluate the extent and mechanism anti-cancer drug-loaded liposomes using wheat germ agglutinin as a guiding molecule.
 Methods: For the drug-loaded liposome synthesis, the thin film hydration method was used and the drug cisplatin was loaded during the synthesis and followed by the surface modification using wheat germ agglutinin (WGA) lectin. The developed system was confirmed based on transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size (PS) analyzer, polydispersity index and Zeta Potential analyzer.
 Results: The results showed the surface modified by liposomes had the particle size 200±5 nm. The wheat germ agglutinin coated on the surface to liposome led to a reduction in zeta potential and drug entrapment efficiency while particle size increased. Plain liposomes containing cisplatin had less effect than WGA modified liposome on MCF-7 cell lines.
 Conclusion: The MTT studies indicated that the drug molecules were initially get delivered to the inside the cell. This formulation offered new simple approach and effectively kill the cells via targeting the nucleus.
Highlights
The cisplatin is the anticancer drug and acts by forming platinum-DNA adducts that hinder rapidly dividing cells from duplicating their DNA for mitosis, lead to activations and enhancement of apoptosis
In the present work cisplatin bearing liposomal drug delivery system was prepared by thin-film hydrations method using PC, CHOL and SA in the ratio of 7:3:1
Platinum compounds are present in the core of cisplatin, carries a 2+or 4+charges, two NH3 groups and two C1 groups that allow efficient liposome encapsulation [16, 17]
Summary
The cisplatin is the anticancer drug and acts by forming platinum-DNA adducts that hinder rapidly dividing cells from duplicating their DNA for mitosis, lead to activations and enhancement of apoptosis. The less cisplatin accumulation in cancer cells may be occurred due to defects in the uptake process, inhibition in drug uptake, an increase in drug efflux. In this regard, some drug delivery based approaches are developed but drug conjugation to nanocarriers, cisplatin provides limited choices. It has been found that the drug encapsulated liposomal system has the potential to protect the stability and integrity of therapeutic molecules and deliver the therapeutic agent to the desired target site Incorporation of this therapeutic molecule in the lipid bilayer is a selfassembly process and helps to reach drug molecule at the target site in control release manner because the local density of certain polymer residues on the liposome surface increases with the protonation of the polymer pendant groups, triggering the collapse of the liposomal membrane and release of its payloads [3]
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