Abstract
SEVERAL bioartificial liver researchers adopt C3A/ HepG2 hepatoma cell line in their designs. Advantages of using this cell line in contrast to rat or porcine hepatocyte primary cells include stability, ease of proliferation, and possibility of long-term maintenance. Although some researchers have carried out comparative studies of C3A/HepG2 with other types of hepatocytes, there are no comprehensive surveys of the characteristics of C3A/ HepG2. Besides, to get a quantitative picture of threedimensional growth of hepatocyte, it is important to first realize the cell growth, toxin production and proteins synthesis kinetics in two-dimensional growth condition. In this study, we analyze the kinetics of concentration changes of ammonia (a key toxin of metabolism and a clinical index of liver detoxification function), albumin, and alpha-fetoprotein (a survival evidence of the hepatocyte) in the culture media, followed by finding out the relationship between cell density and those anabolites by using a linear type expression.
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