Abstract
ABSTRACT Objective: The objective of the present study was to evaluate, in vitro, fibroblastic proliferation on chemically conditioned root surfaces. Methods: Forty single-rooted human teeth, were cut into fragments and divided into four groups (n=20): GI (control) - scaling and root planing (SRP); GII - SRP + conditioning with 10% citric acid; GIII - SRP + conditioning with 24% EDTA gel and GIV - SRP + conditioning with 50 mg/ml of tetracycline hydrochloride. The fibroblasts were placed on these surfaces and cell proliferation evaluated using Trypan Blue stain. Smayer layer formation was analyzed using Scanning Electron Microscopy. Results: The results revealed that the chemical conditioners used were incapable of effectively removing the smear layer. However, when compared to the other groups, GIII showed the best results regarding removal of the smear layer (p<0.05). GI demonstrated the greatest cell proliferation at all studied time intervals compared to the chemically treated groups (p<0.05). Conclusion: The results of the present study reveal that all demineralizing agents evaluated presented lower cell proliferation levels when compared to the control group. In addition none of the chemical conditioners used removed the smear layer completely.
Highlights
Periodontal disease is characterized by a chronic inflammatory process, in which the main agent is the bacterial biofilm[1,2]
The results revealed that the chemical conditioners used were incapable of effectively removing the smear layer
The results of the present study reveal that all demineralizing agents evaluated presented lower cell proliferation levels when compared to the control group
Summary
Periodontal disease is characterized by a chronic inflammatory process, in which the main agent is the bacterial biofilm[1,2]. Root surface conditioning of a tooth affected by periodontal disease aims to remove dental calculus, bacterial biofims and their toxins[2,8,9], which contributes to a reduced inflammatory process, periodontal tissue repair[10,11,12] including formation of new supporting tissues[12,13,14]. An alternative method to remove the smear layer is the combination of debridment and chemical root conditioning, which may be capable of decontaminating, detoxifying and demineralizing the root surface, opening the dentinal tubules and exposing the collagen matrix[2,11,12,13,14,16], which is fundamental for recolonization of cells involved in periodontal repair[2,10,11,12,14], promoting a urface that is biologically compatible with both epithelial and connective issues[2,12,14,17,18]. Many substances have been used with this objective in mind, mainly in solution form, including citric acid, tetracycline and ethylenediaminetetraacetate acid (EDTA)[13]
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