Abstract
Leptospirosis is now acknowledged as the most widespread zoonoses in the world. Hundreds of cases occur in India every year accounting for considerable morbidity and sizable mortality. Several studies have delineated the epidemiology, pathology and variable clinical features of this condition. The present study comprises the importance and utilization of traditional based medicines to overcome the adverse reaction by conventional drugs and standardize the technology. The antileptospiral activity of Eclipta alba L. was well studied by both tube dilution and micro dilution techniques and the result showed better inhibitory action against various serogroups of Leptospira interrogans. L. australis, L. autumnalis and L. grippotyphosa are inhibited by both water and ethanol extract by tube dilution technique. The MIC level observed are 50 μg and 100 μg respectively. Similarly acetone extract, Icterohaemorrhagiae was responded to 200 μg/ml as MIC whereas in petroleum ether extract, no inhibition was observed. In the case of micro dilution technique, the entire inhibition rates are supported to the tube dilution technique. It showed that the micro dilution technique is the best method where we obtained the results within 30 minutes; at the same time tube dilution technique takes minimum of 7 days to provide the result.
Highlights
Leptospirosis is acknowledged as the most widespread zoonoses in the world
Inspite of modern developments medical facilities, about 80% of Indian population are depend on traditional system of medicines because of severe adverse reactions by western medicines[2]
Disease of worldwide in distribution which affects internal organs producing multiple organ dysfunction (MOD) to multiple organ failure (MOF), which is basically an occupational disease; man gets the infection by virtue of his occupation[3]
Summary
The antileptospiral activity of Eclipta alba L. was well studied by both tube dilution and micro dilution techniques and the result showed better inhibitory action against various serogroups of Leptospira interrogans. The efficacy of Eclipta alba against L. interrogans serogroups was investigated by evaluating the minimum inhibitory concentration (MIC) of the extracts like acetone, water and saponiÞed lipid by using standard tube dilution in comparison with micro dilution technique[11]. In water extract under micro dilution technique, the minimum inhibitory concentration like 50 μg showed 100% inhibition against serogroups like Australis, Autumnalis and Grippotyphosa. The acetone extracted Phyllanthus niruri was already studied and proved as best antileptospiral agent but Eclipta alba showed moderate reduction when compared with aqueous and ethanol basis. It is conÞrmed that the bioactive compounds of Eclipta alba L. may be used as an antimicrobial; agent
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