In Vitro Evaluation of Desensitizing Agents Containing Bioactive Scaffolds of Nanofibers on Dentin Remineralization.

Natália Bastos-Bitencourt,Maria Gardennia Da Fonseca,Lucio Castellano,Cassiana Scotti,Juliana Bombonatti,Tatiana Nascimento,Salvatore Sauro,Luiz Goulart,Sergio Ishikiriama,Marilia Velo

doi:10.3390/ma14051056

Abstract

This study evaluated the effect of the incorporation of bioactive nanofibers in desensitizing agents on dentin permeability. Sixty disks of dentin were randomly distributed in four groups (n = 15). Distribution was based on the desensitizing agents, fluoride varnish and self-etching adhesive, and the presence of nanofibers: C (self-etching adhesive Clearfil SE Bond), CN (Clearfil SE Bond with 1% nanofiber), D (Duraphat varnish), and DN (Duraphat varnish with 1% nanofiber). Dentin permeability was determined using hydraulic conductivity. For a qualitative analysis, confocal laser microscopy and scanning electron microscopy were performed. The C group showed the lowest hydraulic conductance (Lp%) (89.33), while the DN group showed the highest Lp% (116.06). No statistical significance was observed in the Lp% values in all groups after the treatment and 6% citric acid challenge (p > 0.239). In the images, the CN group presented a higher superficial and intratubular deposition. In addition, this group presented a more homogeneous dentin surface and wide occlusion of dentinal tubules than the other treatments. Despite there being no statistical differences among the treatments employed, the images showed that the CN group presented a higher surface and intratubular deposition compared to the other treatments, even after the acid challenge.

Highlights

Dentin hypersensitivity (DH) can be classified as a pain symptom arising from exposed dentin, associated or not to noncarious cervical lesions, in response to thermal, mechanical, osmotic, and chemical stimuli [1]
The hydrodynamic theory has shown that even a partial reduction in the functional radii of the dentinal tubules will lead to a reduction in fluid flow, thereby reducing pain symptoms [5]
Sixty dentin discs were obtained from human third molars and were randomized into four groups (n = 15): C, CN (Clearfil SE Bond with 1% nanofibers), D (Duraphat varnish-positive control), and DN (Duraphat varnish with 1% nanofibers)

Summary

Introduction

Dentin hypersensitivity (DH) can be classified as a pain symptom arising from exposed dentin, associated or not to noncarious cervical lesions, in response to thermal, mechanical, osmotic, and chemical stimuli [1]. It is likely to be a more frequent dental complaint in the years owing to the increase in the longevity of dentition [3]. One of the most accepted hypotheses for the mechanism of DH is the hydrodynamic theory reported by Brännström and Aström in 1967 [4]. Under DH, the dentinal tubules are frequently open, leading to an increased dentin permeability. The hydrodynamic theory has shown that even a partial reduction in the functional radii of the dentinal tubules will lead to a reduction in fluid flow, thereby reducing pain symptoms [5]

Methods

Results

Conclusion