Abstract
In this study, a decellularization process was employed to remove the cellular components from small intestine of buffalo ( Bubalus bubalis ) using 2% ionic biological detergent. The acellular matrix then was cross linked with 0.6% glutaraldehyde (GA), 1% 1, 4-butanediol diglycidyl ether (BDDGE) and 1% 1- ethyl- 3 - (3 - dimethylaminopropyl) carbodiimide (EDC). The physical properties of cross linked samples were compared, in vitro , to those of the acellular counterparts on the basis of gross observations, degradation tests, free protein contents, free amino group contents, fixation index, free hydroxyproline contents determination, moisture percent and SDS-PAGE analysis. GA treated tissue showed highest resistance to nonenzymatic, enzymatic degradation and significant reduction (P<0.05) of free protein contents, free amino groups contents and moisture percentage. SDS-PAGE analysis revealed that GA treated acellular small intestinal matrix expressed highest cross-linking, followed by EDC, and BDDGE treated tissues.
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