In Vitro Evaluation of Biological Effects on Human Keratinocytes Exposed to 900 MHz Electromagnetic Field

Abstract

The biological effects of electromagnetic radiation at ultra high frequency on human keratinocytes were investigated. HaCaT cell system, a spontaneously immortalized human keratinocytes cell line, was chosen as model since skin is usually exposed to electromagnetic radiation at considered frequency more than inner tissues. HaCaT cells were exposed to a pure sinusoidal field at a frequency of 900 MHz (average SAR levels ranging from 0.04 to 0.08 W/kg) in plane wave condition inside a GTEM chamber for time intervals of 18 hours at a controlled temperature of 37∘C. Growth curves were obtained for exposed and control cells, and a reduction in the number of exposed cells in comparison to unexposed ones was found; however the number of dead cells was not significantly different in exposed and unexposed samples. In order to investigate whether the reduction in cell proliferation of exposed cells was due to the activation of a differentiative process, the expression of two hallmarks of differentiation was examined. Both keratin 1 and involucrin expression was increased in exposed in comparison to unexposed cells. These results suggest that this protocol of exposure to 900 MHz electromagnetic field can trigger a differentiation program in HaCaT cells.