Abstract
Three polysaccharides namely, exopolysaccharide (EPS), water-extracted mycelial polysaccharide (WPS) and sodium hydroxide-extracted mycelial polysaccharide (SPS), were prepared from the endophytic fungus Berkleasmium sp. Dzf12 isolated from the rhizomes of Dioscorea zingiberensis, and their in vitro antioxidant activities were investigated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, Fe3+ reducing activity, Fe2+ chelating activity, and hydroxyl radical scavenging activity assays. Among these three polysaccharides, EPS and SPS exhibited more excellent antioxidant activities, with corresponding median effective concentration (EC50) values as 61.60 µg/ml and 91.51 µg/ml by using DPPH scavenging assay, 47.82 and 63.91 µg/ml by using reducing Fe3+ assay, 53.37 and 51.27 µg/ml by using chelating Fe2+ assay, and 461.23 and 598.75 µg/ml by using hydroxyl radical scavenging assay, respectively. The results suggested that the polysaccharides, EPS and SPS from the endophytic fungusBerkleasmium sp. Dzf12 could be considered as a promising potential source for the development of natural antioxidants. Key words: Polysaccharides, preparation, in vitro antioxidant activity, endophytic fungus,Berkleasmium sp. Dzf12, Dioscorea zingiberensis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
