Abstract
In VitroEvaluation of Antagonistic Microorganisms for the Control of Die-Back of Neem Causal AgentPhomopsis AzadirachtaeThe die-back of neem caused byPhomopsis azadirachtaeis a devastating disease in India reducing the life span and seed production of neem. Six isolates of antagonistic bacteria and fungi,Bacillus cereus(MTCC 430),B. subtilis(MTCC 619),Pseudomonas aeruginosa(MTCC 2581),P. oleovorans(MTCC 617),Trichoderma harzianum(MTCC 792) andT. viride(MTCC 800) were evaluated againstP. azadirachtaeunderin vitroconditions. Culture filtrates of all these microorganisms were extracted using ethyl acetate, and the obtained fractions were tested for their antifungal activity againstP. azadirachtaeat different concentrations. Ethyl acetate extracts ofB. subtilisandP. aeruginosawere highly effective and completely inhibited the growth ofP. azadirachtaeat 25 ppm concentration. Both these isolates may be considered as factors for the biological control of die-back of neem.
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