Abstract
Euzymic omega oxidation of medium-chain fatty acids, having 8–12 carbon atoms, to their corresponding dicarboxylic acids can be carried out by mammalian tissue homogenates, in vitro. The overall conversion of decanoic acid to decanedioic acid by homogenates of mammalian tissues requires ATP, Mg 2+, DPN +, TPN +, and oxygen. A mixture of liver microsomes (5000 – 25,000 g fraction) and a liver-soluble supernatant fraction (25,000 g supernatant fraction) can replace the whole homogenate in the overall conversion. The omega oxidation of medium-chain fatty acids to their corresponding dicarboxylic acids proceeds through a series of reactions. The first reaction is the direct hydroxylation of the omega methyl group by liver microsomes requiring TPNH and oxygen. The microsomal enzyme system can be called an omega fatty acid hydroxylase. In the second reaction, the omega hydroxy fatty acid is oxidized to the omega oxo fatly acid by a liver-soluble supernatant, fraction requiring DPN +. In the final reaction, the omega oxo fatly acid is oxidized to the dicarboxylic acid by a liver-soluble supernatant fraction, also requiring DPN +. The soluble supernatant enzymes involved in the final two steps of the reaction can be called omega fatty acid dehydrogenases.
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