Abstract
Euzymic omega oxidation of medium-chain fatty acids, having 8–12 carbon atoms, to their corresponding dicarboxylic acids can be carried out by mammalian tissue homogenates, in vitro. The overall conversion of decanoic acid to decanedioic acid by homogenates of mammalian tissues requires ATP, Mg 2+, DPN +, TPN +, and oxygen. A mixture of liver microsomes (5000 – 25,000 g fraction) and a liver-soluble supernatant fraction (25,000 g supernatant fraction) can replace the whole homogenate in the overall conversion. The omega oxidation of medium-chain fatty acids to their corresponding dicarboxylic acids proceeds through a series of reactions. The first reaction is the direct hydroxylation of the omega methyl group by liver microsomes requiring TPNH and oxygen. The microsomal enzyme system can be called an omega fatty acid hydroxylase. In the second reaction, the omega hydroxy fatty acid is oxidized to the omega oxo fatly acid by a liver-soluble supernatant, fraction requiring DPN +. In the final reaction, the omega oxo fatly acid is oxidized to the dicarboxylic acid by a liver-soluble supernatant fraction, also requiring DPN +. The soluble supernatant enzymes involved in the final two steps of the reaction can be called omega fatty acid dehydrogenases.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.