In-vitro endophytic bacteria activity against Ralstonia syzygii subsp. indonesiensis, the causal agent of bacterial wilt disease in tomato (Solanum lycopersicum) to achieve sustainable agriculture

Abstract

The bacterial wilt disease transmitted through soil, identified as Ralstonia syzygii subsp. indonesiensis, is a substantial vascular disease and major constraint in various Solanaceae plants. The use endophytic bacteria-as Arthrobacter sp. and Bacillus thuringiensis-is one approach to control this pathogen. In vitro test using the disc paper method was conducted to evaluate the compatibility of Arthrobacter sp. and B. thuringiensis, along with their inhibitory capabilities through secondary metabolite production, in suppressing the growth of R. syzygii subsp. indonesiensis. The results showed incompatibility among isolates of endophytic bacterial, consequently, both endophytic bacteria were capable suppressing the growth of the R. syzygii subsp. indonesiensis significantly. Arthrobacter sp. formed a larger inhibition zone compared to B. thuringiensis, and both endophytic bacteria can be candidate biological control agents to reduce pesticide use to control R. syzygii subsp. indonesiensis, which therefore achieving more environmentally agriculture sustainability.