Abstract
Embryonic axes and seedling shoot tips of Juglans nitra L., Black walnut, were cultured in vitro. Significant variation existed among progeny from individual trees for growth of radicles and epicotyls and production of callus and axillary shoots from embryonic axes. The concentration of 6-benzyladenine influenced the growth of the radicle and epicotyl and production of callus and axillary shoots of axes. Axes generally initiated growth quicker on solidified woody plant medium than on Driver and Kuniyuki's walnut medium, but axillary shoot proliferation and elongation were eventually better on liquid Driver and Kuniyuki's walnut medium than on woody plant medium which required an etiolation treatment for microshoot elongation. The concentration of BA also influenced both callus growth and axillary shoot proliferation from seedling shoot tips. Axillary shoots which formed in Driver and Kuniyuki's walnut medium rooted best in sterile vermiculite following a 15 s dip in 10 mM indole-3-butyric acid. Micropropagated plants are growing in the greenhouse.
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