In vitro efficacy of triclosan coated polyglactin 910 suture against common bacterial pathogen causing surgical site infection

Abstract

Surgical-site infections (SSIs) increase morbidity and mortality in post-surgical patients as well as represent an economic burden to healthcare systems. The aim of this study was to evaluate the in-vitro efficacy of triclosan coated polyglactin 910 suture against the common bacteria isolated from post-operative wound infection. Our goal was to establish whether the use of a triclosan coated suture would reduce the incidence of microbial colonisation of suture material thus reducing the rate of surgical site infection. Similar length (4cm) of triclosan coated and uncoated sutures were put on the lawn culture made on Mueller Hinton agar by 0.5 McFarland standard suspensions prepared by touching 4-5 colonies of each bacterium isolated from post-operative wound infections. after overnight incubation at 37°C, the zone of inhibition around triclosan coated sutures was compared to the zone of inhibition that was found around uncoated sutures. among 271 (81.87%) positive cultures from 331 post-operative wound samples, the commonest bacterial isolates were Staphylococcus aureus (29.52%), followed by Escherichia coli (17.34%), Klebsiella spp. (15.13%), Coagulase negative Staphylococcus (9.96%), Pseudomonas spp. (9.22%), Proteus spp. (6.64%), Enterococcus spp. (5.53%), Citrobacter spp. (3.69%), and Acinetobacter spp. (2.95%). It was found that after overnight incubation at 37°C, a good zone of inhibition was present around triclosan coated sutures in all isolates except Pseudomonas spp. and Enterococcus spp. – but minimal or no such zone was seen around uncoated sutures. Triclosan coated suture showed good antibacterial activity in-vitro and may be assumed to significantly reduce the SSI rate, cost and duration of hospital stay as it is highly effective in-vitro against the common bacteria isolated from post-operative wound infection.