In vitro effects of triiodothyronine on gene expression in mouse trophoblast cells

Abstract

The objective of the present study was to evaluate the effects of different doses of T3 (10−4 M, 10−7 M, 10−9 M) on the in vitro gene expression of Tpbp, Prl3b1, VEGF, PGF, PL-1, and INFy in mouse trophoblast cells by real-time RT-PCR. Doses of 10−7 and 10−9 M T3 increased the mRNA levels of Tpbp, Pl3b1, VEGF, PGF, INFy and PL-1. In contrast, the dose of 10−4 M reduced the gene expression of PL-1 and VEGF. T3 affected the gene expression of differentiation, hormonal, immune and angiogenic factors in mouse trophoblast cells.