Abstract
The following study was performed to determine if the in vitro stimulatory effect of growth hormone on protein synthesis in the rat diaphragm involves an increase in the number or activity of functional ribosomes in the cells. Isolated diaphragms of hypophysectomized rats were preincubated with bovine growth hormone at 37 C to activate the protein synthetic mechanism. Then they were incubated with 3Hpuromycin, which forms a peptide bond with nascent peptides on the functional ribosomes. Incubation with 3H-puromycin was carried out either in the presence of 1 mM emetine or at 2 C in order to prevent the formation of new nascent peptides during the course of the puromycin reaction. In the presence of emetine, the labeling of nascent peptides with the antibiotic proceeded in a curvilinear fashion and reached completion within 4–6 hr. The rate of labeling at 2 C was much slower. When diaphragms were preincubated for 1 hr with growth hormone and then incubated with 3H-puromycin in the presence of emetine for 6...
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