Abstract
Aim In the present study, we assessed the ability of etanercept to influence the in vitro development of different T cell subsets in JIA patients. In particular, we have evaluated the ability of peripheral blood (PB) and synovial fluid (SF) lymphocytes from patients followed in our Unit, to proliferate and produce cytokines in response to polyclonal (antiCD3/antiCD28) stimulation in presence or in absence of etanercept.
Highlights
Recently, we found that CD4+CD161+ T lymphocytes showing transient nature of the Th17 phenotype are present in the synovial fluid of patients with JIA, and that their accumulation positively correlates with parameters of inflammation, supporting the hypothesis that these cells may play a role in disease activity
Lymphocytes from peripheral blood (PB) and synovial fluid (SF) have been cultured in the presence of etanercept (5 μg/ml), and proliferative responses were evaluated as thimidine uptake and % of bromodeoxyuridine positive cells
AOU Meyer and Careggi, Firenze, Italy stimulation of both PB- and SF-derived total CD4+ lymphocytes, whereas the presence in culture of etanercept increased the frequency of proliferating CD4+CD161+ lymphocytes
Summary
Rolando Cimaz*, Lorenzo Cosmi, Laura Maggi, Gabriele Simonini, Teresa Giani, Ilaria Pagnini, Veronica Santarlasci, Manuela Capone, Valentina Querci, Francesco Liotta, Enrico Maggi, Francesco Annunziato. From 18th Pediatric Rheumatology European Society (PReS) Congress Bruges, Belgium. From 18th Pediatric Rheumatology European Society (PReS) Congress Bruges, Belgium. 14-18 September 2011
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