Abstract
This study sought to determine whether estradiol-17β (E2) could induce proliferation of interstitial cells (fibroblasts and myoid cells) in the immature rainbow trout (Oncorhynchus mykiss) testis. To investigate this, a floating organ culture system was utilized. The results showed that a dose of 18.3n M E2 added to the medium significantly increased interstitial cell proliferation. Pretreatment of the tissue with the estrogen receptor antagonist ICI 182,780 nullifies this proliferative effect. These results show that E2 can enhance interstitial cell proliferation in the immature rainbow trout testis before spermatogenesis begins.
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