In vitro effects of acetaminophen drug(Paracetamol) on microscopic human sperm parameters

Abstract

Paracetamol, also recognized as acetaminophen (CH3CONH.C6H4), is a drug used to relief pain and fever. It is usually used to relief mild to moderate pain . Forty semen samples were taken from forty infertile patients. After semen analysis assessment, The samples were classified into 2 aliquots group, Pre - activation and Post-Activation group. The Post-Activation group was divided into three subgroups: First subgroup G1 (control group) containing semen sample with in vitro Flashing activation medium. Second subgroup G2 containing semen sample with in vitro Flashing activation medium supplemented by Paracetamol drug powder (0.0226 g/l). Third subgroup G3 containing semen sample with in vitro Flashing activation medium supplemented with Paracetamol drug powder (0.0453 g/l). Direct swim up and centrifugation technique was used for sperm activation in vitro . Sperm parameters were assessed and data were analyzed statistically pre- and post-activation in G1,G2 and G3. The results showed a significant increase in the percentage of sperm motility (%) in G1 and G2 groups (P<0.05) in post-activation when compared with pre-activation. However, there was no significant difference in the post-activation sperm total motility (%) between the G1 and G2 groups. Also there is significant difference (P<0.05) of normal sperm morphology observed between Pre- and Post-Activation using Flashing medium supplemented with Paracetamol drug and there is non-significantly different (P>0.05) of post activation of subgroup G1 and G2 and G3. There were significant different (P<0.05) sperm concentration seen between Pre- and Post-activation using Flashing medium supplemented with Paracetamol Drug while there is non-significantly different (P>0.05) of post activation groups compare with each other G1,G2 and G3. The results of this clinical research indicated paracetamol as antipyretic, analgesic drug have no significant (in vitro) effect on sperm motility, concentration, morphology in post activated semen samples. So this drug not preferred to use for increase IVF technique successful rate.