Abstract
The use of tobacco and its products are known to cause many illnesses including cancer. A smokeless tobacco locally manufactured called tuibur (tobacco brew) has been consumed by the Mizos from a very long time. In this experiment we aim to determine the cytotoxicity of tuibur by an in vitro study on tuibur-treated human peripheral blood lymphocytes. We have found that 24 h treatment of human lymphocytes with two grades of commercial tuibur and nicotine showed a concentration dependent decrease in cell viability. We, therefore, concluded that as the in vitro use of tuibur has an adverse effect on cell survival, its consumption might have potential side effects on the health of the users.
Highlights
Tobacco is linked with many diseases and has been known to contain more than eight thousand chemicals, out of which roughly 68 are probable carcinogens.[1,2,3] Some of the common toxic chemicals include benzo[a]pyrene (B[a]P), N’-nitrosonornicotine (NNN), N’nitrosoanatabine (NAT), N’-nitrosoanabasine (NAB), 4-(methylnitrosamino)-1-(3-pyridyl)-1butanone (NNK), N-nitrosodimethylamine (NDMA), nitrite, cadmium, lead, arsenic, nickel, chromium, etc.[4,5] The consumption of both smoking and smokeless tobacco is popular throughout the world and its detrimental effect could be observed from many medical records.Besides its deleterious consequence upon the pulmonary system, it has been linked with many forms of cancer
Lymphocytes treated with a maximum concentration of 50 μl/ mlof tuibur-A and tuibur-B showed 65.57% and 76.14% viability respectively while a minimum concentration of 2.5 μl/ml of both the two tuibur grades resulted in 100% viability in both the groups
A strong negative correlation was observed between cell viability and concentration of tuibur-A (-0.994), tuibur-B (-0.969) and nicotine (-0.979)
Summary
Tobacco is linked with many diseases and has been known to contain more than eight thousand chemicals, out of which roughly 68 are probable carcinogens.[1,2,3] Some of the common toxic chemicals include benzo[a]pyrene (B[a]P), N’-nitrosonornicotine (NNN), N’nitrosoanatabine (NAT), N’-nitrosoanabasine (NAB), 4-(methylnitrosamino)-1-(3-pyridyl)-1butanone (NNK), N-nitrosodimethylamine (NDMA), nitrite, cadmium, lead, arsenic, nickel, chromium, etc.[4,5] The consumption of both smoking and smokeless tobacco is popular throughout the world and its detrimental effect could be observed from many medical records. Besides its deleterious consequence upon the pulmonary system, it has been linked with many forms of cancer. Many studies suggested that almost all known cancer could be linked to tobacco use.[6,7].
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