IN VITRO EFFECT OF RESORCINOL ON BOVINE SPERMATOZOA IN PROCESS OF CRYOPRESERVATION

Agnieszka Greń,Eva Strapáková,Lucia Strapáková,Marko Halo,Martin Massányi,Peter Ivanič,Peter Massányi,Peter Strapák,Tomáš Slanina

doi:10.15414/jmbfs.2020.10.2.325-328

Abstract

The purpose of present study was to observe the effect of resorcinol on bovine spermatozoa before and after cryopreservation. Fresh semen was obtained from six randomly chosen breeding bulls. Experimental samples were prepared by diluting the semen with six different concentrations of resorcinol (REZ1– 4; REZ2 – 2; REZ3 – 1; REZ4 – 0.5; REZ5 – 0.25 and REZ6 –0.152 mg.ml-1). Experimental groups were compared against control group (REZK). Using CASA method spermatozoa motility was evaluated before cryopreservation and also after thawing, during incubation at 37°C in time periods 0, 5, 10, 15 and 60 minutes. Negative effect of in vitro resorcinol addition was observed in all analysed parameters (MOT, PRO, VCL, ALH and BCF) in groups REZ1, REZ2 and REZ3. In contrast, positive impact mainly on motility and progressive motility was found in experimental groups with the lowest resorcinol concentration addition (REZ4, REZ5 and REZ6) in comparison to the control group. After thawing significant effect of resorcinol on motility parameters were observed within each experimental group. Group with the highest concentration of resorcinol (REZ1) had markedly negative impact on evaluated motility parameters. Results of our study clearly confirm toxic effect of resorcinol on motility parameters of spermatozoa, which depend on concentration and time period.

Highlights

Breeding of animals underwent significant changes in the past years
Experimental samples were prepared by diluting the semen with six different concentrations of resorcinol (REZ1– 4; REZ2 – 2; REZ3 – 1; REZ4 – 0.5; REZ5 – 0.25 and REZ6 –0.152 mg.ml-1)
Negative effect of in vitro resorcinol addition was observed in all analysed parameters (MOT, PRO, VCL, ALH and Beat cross frequency (BCF)) in groups REZ1, REZ2 and REZ3

Summary

INTRODUCTION

Breeding of animals underwent significant changes in the past years. Insemination is a process which helped in genetic development of direct and indirect utility attributes and can be marked as one of classic biotechnologies. Insemination is executed using fresh diluted semen or conserved semen. Conservation of semen can be done by chilling or freezing process (Diskin, 2018). Cells are exposed to osmotic and thermic shocks that underway during dilution, cooling-freezing and thawing process. Administration of orally taken resorcinol can be very hazardous on human health (Duran et al, 2004). It has been reported, that resorcinol can induce various toxic effect on central nervous system followed by myocardial depression, central necrosis of liver, tubular and glomerular degeneration of kidney (Thienes and Haley, 1972). The objective of present in vitro study was to evaluate the effect of resorcinol on bull spermatozoa motility parameters before cryopreservation and following thawing

MATERIAL AND METHODS

RESULTS AND DISCUSSION

CONCLUSION