In vitro Effect of Replacement Mineral Salts of Trace Elements with P-containing Chelates to Improve Rooting of Cherry Rootstock (cv. VC-13)

Abstract

In this paper, we studied the effect of modified culture medium based on Murashige and Skoog (Murashige T. Skoog F., 1962) on the in vitro rhizogenesis of clonal rootstock of stone cultures - VC-13. For the first time, the nutrient medium was modified with complexes of all incoming trace elements based on a phosphorus-containing ligand of the bisphosphonate class. The effect of 3 levels of concentrations of chelated trace elements was studied: 1.25, 2.5 and 5 ml/l without the use of increased doses of BMI, and a comparative analysis was carried out in comparison with the modification of trace elements based on EDTA. The maximum effect – 100% rooting after 30 days of subcultivation was found at a concentration of chelated trace elements of 5 ml/l. When modifying EDTA, rooting did not occur. Also, the modification of the nutrient medium by phosphorus-containing complexes contributed to a change in the nature of the formation of the root system of micro-gears. Micro-plants had from 3 to 6 short strong roots, convenient for washing from the nutrient medium and then planting at the stage of adaptation to non-sterile conditions. Repeated tests of the effect of culture medium modification on rhizogenesis in vitro on a larger scale confirmed the high efficiency of this approach. The rooting rate of micro-gears of the VC-13 clone rootstock was more than 80%. The results obtained allow us to conclude about the key role of the nature of the chelating agent.