Abstract
Improving soft tissue attachment to implant abutments is a crucial factor for enduring health and maintenance of soft peri-implant tissue health. In this in vitro study we aimed to compare the biocompatibility of three different abutment surfaces: titanium, zirconia and modified polyetheretherketone (PEEK). Surface topography, roughness and wettability were investigated with scanning electron microscopy, profilometer and contact angle meter, respectively. Human gingival epithelial keratinocytes were examined for viability, morphology, proliferation and migration by using tetrazolium salt colorimetric assay, scanning electron microscopy imaging, immunofluorescence bromodeoxyuridine analysis and scratch wound healing assays. Roughness measurements revealed differences between the investigated surfaces. Keratinocytes cultured on all examined surfaces indicated adhesion and attachment by means of scanning electron microscopy imaging. Cell viability assays showed no significant differences between the groups (p > 0.05). The modified PEEK surface similarly improved surface roughness in comparison to titanium and zirconia, which resulted in greater and equivalent cell proliferation and migration. The study methodology showed here may emphasize the importance of cell interactions with different abutment materials, which in part increases the changes of implant success. PEEK, titanium and zirconia surface types used in this study showed mostly similar epithelial biological responses.
Highlights
A central issue in long-term dental implant success is closely related to the integrity of osseointegration, and epithelium health and the quality of attachment of the connective tissue to the implant abutment surface [1,2]
The results demonstrated significant differences concerning in cell viability and migration ability of gingival fibroblasts and oral keratinocytes [14,16]
The macrostructure, microstructure and nanostructure images of the different materials disc surfaces were obtained by using scanning electron microscopy (SEM, FIB-scanningelectron electron microscopy microscopy (SEM) 40 CrossBeam Zeiss Auriga, Carl Zeiss, Jena, Germany) at 1k× and 100k×
Summary
A central issue in long-term dental implant success is closely related to the integrity of osseointegration, and epithelium health and the quality of attachment of the connective tissue to the implant abutment surface [1,2]. Good marginal fit between implant and abutment is important to prevent the bacterial microleakage that may interfere with peri-implant tissue health [3]. Numerous experimental studies with different cell types have been used to analyze cell behavior response towards biomaterials and dental implant abutment surfaces [4,5,6]. Many studies have focused on human gingival epithelial keratinocytes (HGEK) as important cells associated with soft tissue interaction with implant attachment and lining [7,8,9,10].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
