Abstract
Objective To evaluate the in vitro effect of heat treatment on the secretion of a-melanocytestimulating hormone （oL-MSH） by human melanocytes and HaCaT keratinocytes. Methods Cultured normal human melanocytes from forskin tissue and HaCaT cells were treated with hyperthermia at 42 ℃for 1 hour per day for 3 days. Subsequently, real time fluorescence-based quantitative PCR and enzyme-linked immunosorbent assay （ELISA） were performed to detect the mRNA and protein expressions of a-MSH, respectively. Results The mRNA expression level of a-MSH was significantly higher in heat-treated melanocytes and HaCaT cells than in those untreated （1.5862 ±0.3262 vs. 1.0000± 0.2715, P 〈 0.05; 1.8013 ± 0.1216 vs. 1.0000 ± 0.2532, P 〈 0.05）. Moreover, ELISA showed a statistical increase in the supernatant level of a-MSH protein in heat-treated melanoeytes and HaCaT cells compared with those untreated （0.3142± 0.0469 vs. 0.2557 ± 0.0330, P 〈 0.05; 0.4632 ± 0.0345 vs. 0.4389 ± 0.0399, P 〈 0.05）. Conclusion Heat treatment can promote the secretion of a- MSH by melanocytes and HaCaT cells. Key words: Heat; Melanocytes; HaCaT cells; a-MSH
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