Abstract
The aim of this study was to evaluate the in vitro effects of selected pure naturally occuring biologically active compounds (resveratrol-RES, quercetin-QUE, curcumin-CUR, epicatechin-EPI, isoquercitrin-ISO) on rabbit sperm motility using the IDENT staining method and the computer-aided sperm (CASA) analysis. Semen samples were collected regularly from 10 male rabbits, pooled, the seminal plasma was removed and the sperm fraction was exposed to different concentrations (1, 5, 10, 50 and 100 μmol/L) of chosen biomolecules. At culture times of 0h, 2h, 4h, 6h and 8h, the spermatozoa motility was assessed using the TOX IVOS II. CASA system and the IDENT fluorescent staining method. The motility assessment revealed different behavior patterns, specific and unique to each of the studied biomolecules. After 8h of in vitro culture, the highest sperm motility was detected in experimental groups subjected to 10 μmol/L RES (P
Highlights
Current evidence suggests that oxidative stress (OS) represents an important factor in male reproductive dysfunction
The computeraided sperm analysis (CASA) assessment revealed a continuous decrease of spermatozoa motility and in all groups over the course of a 8h in vitro culture (Table 1)
The initial (Time 0h) MOT was higher in the experimental groups supplemented with 5 and 50 μmol/L RES when compared to the Control group, without any statistical significance (P>0.05)
Summary
Current evidence suggests that oxidative stress (OS) represents an important factor in male reproductive dysfunction. A number of reports have emphasized on the positive effects of oral antioxidant administration on male fertility in animals and humans (Donnely et al, 1999; Agarwal and Sekhon, 2010). The in vitro data are important, as it has been shown on numerous occasions that external antioxidants may protect spermatozoa against oxidative injury and a subsequent dysfunction. Such knowledge is essential for spermatozoa processing protocols performed in medical or veterinary andrology for spermatozoa cryopreservation or assisted reproductive technologies (Saleh and Agarwal, 2002). We have validated the fluorescent-based rabbit sperm motility assessment using the IDENT staining method and the computer-aided sperm (CASA) analysis
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