Abstract
The effect of cadmium exposure was studied in vitro on the ATPase activities of gill membrane microsomes from seawater- and freshwater-adapted eel ( Anguilla anguilla) using a microassay technique. The basal activity (Mg 2+-ATPase) was decreased by 40 and 25%, respectively, in seawater and freshwater preparations for the highest concentrations tested (respectively, 4 and 2 μ M). The Na +/K +-ATPase activity was estimated either by potassium stimulation or by ouabain inhibition. This enzyme activity was inhibited by cadmium in a dose-dependent manner with a I 50 of 146 ± 9.3 n M. Neither the technique used to measure the enzyme nor the adaptative environment significantly changed the I 50. The results are compared to results obtained in other groups and to the effects of cadmium on metal ion exchanges in fish.
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